The detection of SARS-CoV-2 using reverse transcription loop-mediated isothermal amplification (RT-LAMP) in developing country

Muhammad Rizky Wibowo; Erna Harfiani; Sarmoko Sarmoko; Yudhi Nugraha

doi:10.51511/pr.9

Muhammad Rizky Wibowo Faculty of Medicine, Universitas Pembangunan Nasional Veteran Jakarta, Indonesia
Erna Harfiani Faculty of Medicine, Universitas Pembangunan Nasional Veteran Jakarta, Indonesia https://orcid.org/0000-0003-4244-3021
Sarmoko Sarmoko Department of Pharmacy, Faculty of Health Sciences, Universitas Jenderal Soedirman, Indonesia https://orcid.org/0000-0002-1315-6085
Yudhi Nugraha Faculty of Medicine, Universitas Pembangunan Nasional Veteran Jakarta, Indonesia https://orcid.org/0000-0003-1186-4093

Keywords: covid-19, reverse transcription loop mediated isotermal amplification, severe acute respiratory syndrome coronavirus-2

Abstract

The severe acute respiratory syndrome coronavirus (SARS-CoV-2) has infected the human system resulting in Covid-19, and has spread rapidly worldwide. Therefore, a fast, simple, cost-effective, and accurate detecting tool is required. The standard diagnostic tool of the World Health Organization is the reverse transcription-polymerase chain reaction (RT-PCR). This method detects the presence of viral genetic material in the human body with accurate results. However, it has several limitations in terms of equipment, personnel, duration, and cost. Therefore, a fast, simple, and sensitive alternative detection, is required, one of which is the reverse transcription loop-mediated isothermal amplification (RT-LAMP) that functions under isothermal conditions. This method is battery-driven, hence, easy to move closer to the patient. Conclusively, the RT-LAMP test for SARS CoV-2 diagnosis produces comparable sensitivity to a standard RT-PCR and is more suitable for resource-poor settings, such as rural areas of developing countries.

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